Peptide ligands to human immunodeficiency virus type 1 gp120 identified from phage display libraries.
Identifieur interne : 003791 ( Main/Exploration ); précédent : 003790; suivant : 003792Peptide ligands to human immunodeficiency virus type 1 gp120 identified from phage display libraries.
Auteurs : M. Ferrer [États-Unis] ; S C HarrisonSource :
- Journal of virology [ 0022-538X ] ; 1999.
Descripteurs français
- KwdFr :
- Anticorps antiviraux (métabolisme), Anticorps monoclonaux (métabolisme), Bactériophage M13, Banque de peptides, Données de séquences moléculaires, Humains, Ligands, Peptides (métabolisme), Protéine d'enveloppe gp120 du VIH (métabolisme), Rayons ultraviolets, Réactifs réticulants, Sites de fixation, Séquence d'acides aminés, VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (métabolisme).
- MESH :
- métabolisme : Anticorps antiviraux, Anticorps monoclonaux, Peptides, Protéine d'enveloppe gp120 du VIH, VIH-1 (Virus de l'Immunodéficience Humaine de type 1).
- Bactériophage M13, Banque de peptides, Données de séquences moléculaires, Humains, Ligands, Rayons ultraviolets, Réactifs réticulants, Sites de fixation, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence, Antibodies, Monoclonal (metabolism), Antibodies, Viral (metabolism), Bacteriophage M13, Binding Sites, CD4 Antigens (metabolism), Cross-Linking Reagents, HIV Envelope Protein gp120 (metabolism), HIV-1 (metabolism), Humans, Ligands, Molecular Sequence Data, Peptide Library, Peptides (metabolism), Ultraviolet Rays.
- MESH :
- chemical , metabolism : Antibodies, Monoclonal, Antibodies, Viral, CD4 Antigens, HIV Envelope Protein gp120, Peptides.
- metabolism : HIV-1.
- Amino Acid Sequence, Bacteriophage M13, Binding Sites, Cross-Linking Reagents, Humans, Ligands, Molecular Sequence Data, Peptide Library, Ultraviolet Rays.
Abstract
We have used phage-displayed peptide libraries to identify novel ligands to the human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein gp120. Screening of libraries of random 12-mers, 7-mers, and cyclic 9-mers produced two families of gp120 binding peptides. Members of a family with the prototype sequence RINNIPWSEAMM (peptide 12p1) inhibit the interaction between gp120 and both four-domain soluble CD4 (4dCD4) and monoclonal antibody (MAb) 17b, a neutralizing antibody that covers the chemokine receptor binding surface on gp120. Peptide 12p1 inhibits the interaction of 4dCD4 with gp120 from three different HIV strains, implying that it binds to a conserved site on gp120. Members of a second family of peptides, with the prototype sequence TSPYEDWQTYLM (peptide 12p2), bind more weakly to gp120. They do not detectably affect its interaction with 4dCD4, but they enhance its binding to MAb 17b. A common sequence motif in the two peptide families and cross-competition for gp120 binding suggest that they have overlapping contacts. Their divergent effects on the affinity of gp120 for MAb 17b may indicate that their binding stabilizes distinct conformational states of gp120. The functional properties of 12p1 suggest that it might be a useful lead for the development of inhibitors of HIV entry.
PubMed: 10364331
Affiliations:
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Le document en format XML
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Ferrer</name><affiliation wicri:level="4"><nlm:affiliation>Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138</wicri:regionArea><orgName type="university">Université Harvard</orgName><placeName><settlement type="city">Cambridge (Massachusetts)</settlement><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Harrison, S C" sort="Harrison, S C" uniqKey="Harrison S" first="S C" last="Harrison">S C Harrison</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1999">1999</date><idno type="RBID">pubmed:10364331</idno><idno type="pmid">10364331</idno><idno type="wicri:Area/PubMed/Corpus">002630</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002630</idno><idno type="wicri:Area/PubMed/Curation">002630</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002630</idno><idno type="wicri:Area/PubMed/Checkpoint">002476</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002476</idno><idno type="wicri:Area/Ncbi/Merge">000019</idno><idno type="wicri:Area/Ncbi/Curation">000019</idno><idno type="wicri:Area/Ncbi/Checkpoint">000019</idno><idno type="wicri:doubleKey">0022-538X:1999:Ferrer M:peptide:ligands:to</idno><idno type="wicri:Area/Main/Merge">003835</idno><idno type="wicri:Area/Main/Curation">003791</idno><idno type="wicri:Area/Main/Exploration">003791</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Peptide ligands to human immunodeficiency virus type 1 gp120 identified from phage display libraries.</title><author><name sortKey="Ferrer, M" sort="Ferrer, M" uniqKey="Ferrer M" first="M" last="Ferrer">M. Ferrer</name><affiliation wicri:level="4"><nlm:affiliation>Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138</wicri:regionArea><orgName type="university">Université Harvard</orgName><placeName><settlement type="city">Cambridge (Massachusetts)</settlement><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Harrison, S C" sort="Harrison, S C" uniqKey="Harrison S" first="S C" last="Harrison">S C Harrison</name></author></analytic><series><title level="j">Journal of virology</title><idno type="ISSN">0022-538X</idno><imprint><date when="1999" type="published">1999</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Antibodies, Monoclonal (metabolism)</term><term>Antibodies, Viral (metabolism)</term><term>Bacteriophage M13</term><term>Binding Sites</term><term>CD4 Antigens (metabolism)</term><term>Cross-Linking Reagents</term><term>HIV Envelope Protein gp120 (metabolism)</term><term>HIV-1 (metabolism)</term><term>Humans</term><term>Ligands</term><term>Molecular Sequence Data</term><term>Peptide Library</term><term>Peptides (metabolism)</term><term>Ultraviolet Rays</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps antiviraux (métabolisme)</term><term>Anticorps monoclonaux (métabolisme)</term><term>Bactériophage M13</term><term>Banque de peptides</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Ligands</term><term>Peptides (métabolisme)</term><term>Protéine d'enveloppe gp120 du VIH (métabolisme)</term><term>Rayons ultraviolets</term><term>Réactifs réticulants</term><term>Sites de fixation</term><term>Séquence d'acides aminés</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Antibodies, Viral</term><term>CD4 Antigens</term><term>HIV Envelope Protein gp120</term><term>Peptides</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>HIV-1</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Anticorps antiviraux</term><term>Anticorps monoclonaux</term><term>Peptides</term><term>Protéine d'enveloppe gp120 du VIH</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Bacteriophage M13</term><term>Binding Sites</term><term>Cross-Linking Reagents</term><term>Humans</term><term>Ligands</term><term>Molecular Sequence Data</term><term>Peptide Library</term><term>Ultraviolet Rays</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Bactériophage M13</term><term>Banque de peptides</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Ligands</term><term>Rayons ultraviolets</term><term>Réactifs réticulants</term><term>Sites de fixation</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have used phage-displayed peptide libraries to identify novel ligands to the human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein gp120. Screening of libraries of random 12-mers, 7-mers, and cyclic 9-mers produced two families of gp120 binding peptides. Members of a family with the prototype sequence RINNIPWSEAMM (peptide 12p1) inhibit the interaction between gp120 and both four-domain soluble CD4 (4dCD4) and monoclonal antibody (MAb) 17b, a neutralizing antibody that covers the chemokine receptor binding surface on gp120. Peptide 12p1 inhibits the interaction of 4dCD4 with gp120 from three different HIV strains, implying that it binds to a conserved site on gp120. Members of a second family of peptides, with the prototype sequence TSPYEDWQTYLM (peptide 12p2), bind more weakly to gp120. They do not detectably affect its interaction with 4dCD4, but they enhance its binding to MAb 17b. A common sequence motif in the two peptide families and cross-competition for gp120 binding suggest that they have overlapping contacts. Their divergent effects on the affinity of gp120 for MAb 17b may indicate that their binding stabilizes distinct conformational states of gp120. The functional properties of 12p1 suggest that it might be a useful lead for the development of inhibitors of HIV entry.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Massachusetts</li></region><settlement><li>Cambridge (Massachusetts)</li></settlement><orgName><li>Université Harvard</li></orgName></list><tree><noCountry><name sortKey="Harrison, S C" sort="Harrison, S C" uniqKey="Harrison S" first="S C" last="Harrison">S C Harrison</name></noCountry><country name="États-Unis"><region name="Massachusetts"><name sortKey="Ferrer, M" sort="Ferrer, M" uniqKey="Ferrer M" first="M" last="Ferrer">M. Ferrer</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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